2/13/2023 0 Comments Cool flame co2 maximizerThese observations suggest that the intron(s) or the terminator sequence of the maize gene, or a combination of both, is necessary for high-level expression. In contrast, introduction of a chimeric gene containing the full-length cDNA of the maize PPDK fused to the maize C( 4)-Pdk promoter or the rice Cab promoter only increased PPDK activity and protein level slightly. In a homozygous line, the PPDK protein accounted for 35% of total leaf-soluble protein or 16% of total leaf nitrogen. When the intact maize C( 4)-Pdk gene, containing its own promoter and terminator sequences and exon/intron structure, was introduced, the PPDK activity in the leaves of some transgenic lines was greatly increased, in one line reaching 40-fold over that of wild-type plants. The C( 4)-Pdk gene encoding the C( 4) enzyme pyruvate, orthophosphate dikinase (PPDK) of maize (Zea mays cv Golden Cross Bantam) was introduced into the C(3) plant, rice (Oryza sativa cv Kitaake). Significant accumulation of C( 4)-specific pyruvate, orthophosphate dikinase in a C(3) plant, rice.įukayama, H Tsuchida, H Agarie, S Nomura, M Onodera, H Ono, K Lee, B H Hirose, S Toki, S Ku, M S Makino, A Matsuoka, M Miyao, M
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